Composite

Part:BBa_K2073008

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


fac-dex1 with IPTG promoter and ter

This part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073000(fac-dex1)+BBa_B0012(ter). This biobrick can be transform into E.coli, then the E.coli can express fac-dex1. fac-dex1 is a kind of fluoroacetate dehalogenase. The enzyme can cleavage of a carbonfluorine bond in fluoroacetate to form glycolate. The reaction: a haloacetate + H2O ⇌ glycolate + a halide anion + H+

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
    Illegal AgeI site found at 427
    Illegal AgeI site found at 551
    Illegal AgeI site found at 770
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None